Journal: Scientific Reports

Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies

doi: 10.1038/s41598-025-04291-y

Figure Lengend Snippet: Immunohistochemical labelling of Alzheimer’s disease neuropathology. Immunohistochemical staining was performed as described in the “Materials and methods”. (a) Staining of AD and control frontal cortex tissue using monoclonal antibodies 7F2, AT180, 1F10, and AT100 is shown. Thin arrows indicate NFTs, arrow heads demonstrate neuritic plaques, thick arrows highlight neuropil threads. Scale bar of low magnification images is 100 μm, inset is 15 μm. (b) Semi-quantitative analysis of AD tau pathology in the frontal cortex is described in the “Materials and methods”. Data is presented as mean +/- SEM.

Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr231 , was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Immunohistochemical staining, Staining, Control, Bioprocessing

Journal: Scientific Reports

Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies

doi: 10.1038/s41598-025-04291-y

Figure Lengend Snippet: Immunohistochemical labelling of PSP and CBD neuropathology. Immunohistochemical staining was performed as described in the “Materials and methods”. (a) Staining of PSP and CBD striatal tissue using monoclonal antibodies 7F2, AT180, and 1F10 is shown. Scale bar is 80 μm. (b) Semi-quantitative analysis of PSP and CBD tau pathology in the caudate and putamen is described in the “Materials and methods”. Data is presented as mean +/- SEM.

Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr231 , was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Immunohistochemical staining, Staining, Bioprocessing

Journal: Scientific Reports

Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies

doi: 10.1038/s41598-025-04291-y

Figure Lengend Snippet: Immunohistochemical labelling of PiD neuropathology. Immunohistochemical staining was performed as described in the “Materials and methods”. Staining of PiD hippocampal tissue using antibodies 7F2, AT180, and 1F10 is shown. Scale bar of low magnification images is 100 μm, inset is 15 μm.

Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr231 , was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Immunohistochemical staining, Staining

Journal: NPJ Vaccines

Article Title: pS396/pS404 (PHF1) tau vaccine outperforms pS199/pS202 (AT8) in rTg4510 tauopathy model

doi: 10.1038/s41541-025-01147-4

Figure Lengend Snippet: a Western blot of soluble hippocampal lysates from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice were evaluated for markers of phosphorylated (AT8, AT180, PHF1) and total tau (Tau5). All samples were run on the same gel. b Compared to Qβ Control vaccinated mice, Qβ-PHF1 vaccinated mice showed a significant reduction in pathological AT180 + tau without any reduction in total physiologic tau. c . Immunohistochemistry of brain sections from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice staining for AT180 tau pathology in the cerebral cortex (CTX) and CA1 hippocampus. d There was a significant reduction in AT180 + tau pathology in both the cortex and hippocampus of Qβ-PHF1 vaccinated mice compared to Qβ Control mice ( e , f ). Western blot of soluble hippocampal lysates from Qβ Control and Qβ-AT8 vaccinated rTg4510 mice showed no significant reductions in pathological tau. All graphs display mean ± SEM. Student’s t -test ( b , d , f ). p < 0.05*; p < 0.001***; p < 0.0001****. Qβ Control ( n = 5), Qβ-AT8 ( n = 7), Qβ-PHF1 ( n = 8).

Article Snippet: Antibody dilutions were as follows: AT180 (Thermo Fisher Scientific, MN1040) at 1:500, AT8 (Thermo Fisher Scientific, MN1020) at 1:500, Iba1 (WAKO, 019-19741) at 1:500, and CD45 (BioRad, MCA43R) at 1:250 incubated overnight at 4 °C.

Techniques: Western Blot, Control, Immunohistochemistry, Staining